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KMID : 0361620100450010065
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Journal of the Korean Orthopaedic Association
2010 Volume.45 No. 1 p.65 ~ p.72
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Gene Expression in the Capsule Tissue of the Frozen Shoulder
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Kim Jung-Man
Kim Yang-Soo
Ji Jong-Hun
Lee Jae-Young
Yoo Hyun-Ho
Lee Youn-Kyoung
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Abstract
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Purpose: To investigate the overall expression of extracellular matrix (ECM) and adhesion molecule genes using a gene array technique in the joint capsule of a frozen shoulder.
Materials and Methods: Tissues from 20 human shoulder joint capsules were harvested intraoperatively from patients (15 primary frozen shoulders, 5 controls) in our hospital. The RNA was isolated from the capsule tissue and the gene expression of ECM and adhesion molecules was analyzed using an oligo-array technique.
Results: The expression of several genes of the ECM and cell adhesion molecules was significantly higher in the capsule tissue from patients with a frozen shoulder than the controls. The gene expression of the collagen V α1/α3, VI α2/α3, VIII α1/α2, XV α1, XVIII α1 and ECM proteins including CD 44, connective tissue growth factor (CTGF), matrix metalloproteinase (MMP)-9/14, osteonectin, veriscan, hyaluronan synthase (HAS)-1, extra-cellular matrix (ECM)-1, secreted phosphoprotein (SSP)-1, tenascin C (TNC), thrombospondin 2/4 was two times higher in the frozen shoulder than the control. Several cell adhesion molecules genes including catenin α1, seletin p, integrin α3, β2, β4, β5 and laminin α4, α5 were also two times higher in the in the patients with a frozen shoulder than the control.
Conclusion: The gene expression of several ECM proteins and adhesion molecules is significantly higher in patients with frozen shoulder.
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KEYWORD
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frozen shoulder, oligo-array, extracellular matrix, adhesion molecule
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